Rapid Canine Brucella Ab Kit size: 10 Tests
- Rapid Canine Brucella Ab Kit for canine brucellosis.
Use a 10-test kit.
Materials Needed
- Rapid Canine Brucella Ab Kit (10 tests)
- Blood samples (serum or plasma) from the dogs to be tested
- Disposable pipettes or dropper
- Timer or stopwatch
Procedure
1. Preparation:
- Ensure all components of the kit and samples are at room temperature before beginning the test.
- Wash your hands and wear gloves to maintain hygiene and avoid contamination.
2. **Sample Collection:**
- Collect blood samples from the dogs and separate the serum or plasma. This usually involves drawing blood into a collection tube and allowing it to clot, followed by centrifugation to separate the serum.
3. **Test Setup:**
- Lay out the test cassettes on a flat surface. Ensure each cassette is labeled if testing multiple samples.
4. **Adding the Sample:**
- Using a disposable pipette or dropper, add the specified amount of serum or plasma (usually a few drops) to the sample well on the test cassette. Be sure to follow the kit’s instructions on the exact volume required.
5. **Buffer Addition:**
- Add the required number of drops of buffer solution to the well, as indicated in the kit’s instructions.
6. **Incubation:**
- Allow the test cassette to sit undisturbed for the amount of time specified in the instructions (usually around 10-15 minutes). Use a timer to ensure accurate timing.
7. **Reading Results:**
- After the incubation period, read the results. Most rapid test cassettes have a control line (C) and a test line (T).
- **Positive Result:** Both the control line (C) and the test line (T) appear.
- **Negative Result:** Only the control line (C) appears.
- **Invalid Result:** If the control line (C) does not appear, the test is invalid, and it should be repeated with a new test cassette.
8. **Disposal:**
- Properly dispose of all used test components, including test cassettes, pipettes, and any other materials, according to local regulations for biohazardous waste.
### Notes
- Ensure the control line appears for each test. If it does not, the test is invalid.
- Avoid cross-contamination by using a new pipette or dropper for each sample.
- Consult the kit’s specific instructions for any variations or additional steps.
Rapid Canine Brucella Ab Kit for canine brucellosis.